Page 19 - Analityka 1/2017

Untargeted analysis of Praktyka
phospholipids in biological
samples and foodstuffs

PART I. EXTRACTION OF LIPID FRACTIONS AND BASIC ANALYTICAL TECHNIQUES IN
ANALYSIS OF PHOSPHOLIPIDS

Phospholipids play an important role in the proper functioning of the human body. Therefore, their daily delivery
with food is recommended. Since the biological activity of phospholipids is determined by their chemical structure,
it is necessary to use analytical techniques to determine the full composition of phospholipids present in food, tak-
ing into account not only the phospholipid classes dependent on the structure of hydrophilic moiety but also the
kind of the fatty acids found in the structure. Determination of biological effects of lipid compounds present in the
human body requires a knowledge of their composition, which may be accomplished by targeted and untargeted
analysis. Currently, a number of analytical techniques are used for the qualitative and quantitative determination of
the phospholipid classes. These method embrace: one- and two-dimensional TLC or normal phase and hydrophilic
interaction HPLC. The determination of fatty acids present in individual phospholipid classes is possible with the
use of reverse phase HPLC and gas chromatography.

ANALITYKA – NUMER 1, ROK (XVIII) 2017 49